Continuous microfluidic mechano-poration of EVs for versatile cargo loading

Mechanoporation : Enabling Exosomes Enrichment

In addition to their natural anti-inflammatory and regenerative activity, exosomes can be enhanced by loading an additional payload. A robust and scalable  absorption technology, while maintaining their integrity, is invaluable to a bright future for RNA-enhanced exosomes.

conveyxo leverage a unique mechanoporation solution. It allows continuous and efficient loading, based on making the exosomes temporary porous by squeezing them when circulating through microchip’s channels.

Mechanoporation Unique Benefits

Versatile Cargo Types

Different drug substances can be loaded using the platform. The process has been validated with miRNA, proteins, and water-soluble therapeutic compounds.

Continuous Loading

All exosomes in the solution eventually pass through the chip’s nano-channels, with a maximum of one or two per channel at a time.

Efficiency

The efficiency is around 10-fold higher compared to passive loading. The chips can be engineered to further increase loading efficiency with additional manipulations.

Automation

The microfluidic format is inherently highly compatible with a continuous process format and automation, which will positively impact product quality.

A Scalable Solution

The platform consists of two components:

  • A single chip capable of the small-scale production of GLP-grade material. This chip makes it possible to rapidly test the loading of different drug candidates, producing sufficient material for preclinical characterization.
  • An integrated scalable device for the automated large-scale production of GMP-grade therapeutic exosomes. This device will enable the production of sufficient quantities needed for clinical studies and beyond.

Would you like to learn more about our platform, or are you interested in collaborating? Get in touch!